scholarly journals Control of neuroblastoma cell proliferation and differentiation by human bone marrow

Cancer ◽  
1996 ◽  
Vol 77 (12) ◽  
pp. 2614-2621 ◽  
Author(s):  
Talia Hahn ◽  
Reuven Or ◽  
Gernot Bruchelt ◽  
Lea Malach ◽  
Yocheved Karov ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Proliferation ◽  
Neuroblastoma Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation

scholarly journals Effect of titanium surface roughness on human bone marrow cell proliferation and differentiation: an experimental study

2009 ◽  
Vol 24 (3) ◽  
pp. 200-205 ◽  
Author(s):  
Taís Somacal Novaes Silva ◽  
Denise Cantarelli Machado ◽  
Christian Viezzer ◽  
Aurelício Novaes Silva Júnior ◽  
Marília Gerhardt de Oliveira
Keyword(s):  
Bone Marrow ◽  
Surface Roughness ◽  
Cell Proliferation ◽  
Marrow Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Human Donor ◽  
Proliferation And Differentiation ◽  
Titanium Surfaces ◽  
Intermediate Surface

PURPOSE: To assess the proliferation and differentiation of human bone marrow-derived cells cultured on titanium surfaces with different roughness characteristics. METHODS: Cells obtained from the iliac crest of an adult human donor were routinely processed and cultured on titanium surfaces of varying roughness, according to their preparation method: polishing only (smooth surface) and polishing followed by etching with HF/HNO3 for 15 and 30 minutes (rough surfaces). Surfaces were assessed using scanning electronic microscopy and profilometry. RESULTS: Titanium disks etched with acid for 15 minutes allowed greater cell proliferation in all culture periods. The level of osteopontin and osteocalcin expression was increased in both acid-etched groups, which indicates an advanced stage of differentiation of cells into osteoblasts. CONCLUSIONS: Increased surface roughness accelerates the differentiation of undifferentiated mesenchymal cells into osteogenic lineage cells, but does not necessarily favor cell proliferation. An intermediate surface roughness of 0.5µm (acid etching for 15 minutes) favors both initial and final cell responses.

scholarly journals Long-term human bone marrow cultures

Blood ◽  
1980 ◽  
Vol 56 (1) ◽  
pp. 118-124
Author(s):  
WG Hocking ◽  
DW Golde
Keyword(s):  
Bone Marrow ◽  
Culture System ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Murine Bone Marrow ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures

Pronlonged in vitro growth of murine bone marrow has been achieved using a flask culture system. We report the adaption of the technique to the growth of human bone marrow. In this system, CFU-GM and CFU-E are maintained for 4–9 wk. Morphologically recognizable granulopoiesis occurs for 4–6 wk and erythropoiesis for 1.5–2.5 wk. Functional T lymphocytes are maintained for at least 5 wk. The adherent population is composed of macrophages, fibroblast-like cells, and flat pavement- like cells. Fat-containing cells are not prominant. This culture system provides a means to study human hematopoietic cell proliferation and differentiation in vitro, as well as to examine interactions between stromal cells and hematopoietic and lymphoid progenitors.

Effects of Silver Nanoparticles on the In Vitro Culture and Differentiation of Human Bone Marrow-Derived Mesenchymal Cells

2016 ◽  
Vol 852 ◽  
pp. 1307-1312
Author(s):  
Xiao Qian Yu ◽  
Li Xin Xu
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Silver Nanoparticles ◽  
Antibacterial Properties ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Marrow Mesenchymal Stem Cells

The antibacterial properties of silver nanoparticles may be able to prevent inflammation around an implant when placed on the surface of the dental neck. The purpose of this study was to culture silver nanoparticles on human bone marrow stem cells in vitro to determine their effects on cell differentiation and to assess their biocompatibility. Silver nanoparticles were deposited on titanium foil implant surfaces using ion sputtering, and adult bone marrow mesenchymal stem cells were cultured on those surfaces to determine the effect of the silver nanoparticles on cell viability, suitability for subculture, proliferation and differentiation. The results indicated that the silver nanoparticles were biocompatible, allowing cell proliferation and differentiation of osteoblasts, but interfered with differentiation into chondrocytes and adipocytes.

scholarly journals Long-term human bone marrow cultures

Blood ◽  
1980 ◽  
Vol 56 (1) ◽  
pp. 118-124 ◽  
Author(s):  
WG Hocking ◽  
DW Golde
Keyword(s):  
Bone Marrow ◽  
Culture System ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Murine Bone Marrow ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Bone Marrow Cultures ◽  
Human Bone Marrow Cultures

Abstract Pronlonged in vitro growth of murine bone marrow has been achieved using a flask culture system. We report the adaption of the technique to the growth of human bone marrow. In this system, CFU-GM and CFU-E are maintained for 4–9 wk. Morphologically recognizable granulopoiesis occurs for 4–6 wk and erythropoiesis for 1.5–2.5 wk. Functional T lymphocytes are maintained for at least 5 wk. The adherent population is composed of macrophages, fibroblast-like cells, and flat pavement- like cells. Fat-containing cells are not prominant. This culture system provides a means to study human hematopoietic cell proliferation and differentiation in vitro, as well as to examine interactions between stromal cells and hematopoietic and lymphoid progenitors.

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